| 释义 |
Definition of dewax in English: dewaxverbdiːˈwaks [with object]Remove wax from. 使脱蜡；为…脱蜡 linoleum needs to be dewaxed using a strong solvent 需要一种强效溶剂给油地毡脱蜡。 Example sentencesExamples - Paraffin sections were dewaxed in xylene, rehydrated through a series of graded alcohols, placed in 10mM citrate buffer, and submitted to heat retrieval using a vapor lock for 40 minutes.
- Paraffin tissue sections were placed on silane-coated slides, dewaxed, digested with proteinase K, and then hybridized to digoxigenin-labeled riboprobes.
- Slides were then dewaxed and blocked with endogenous peroxidase.
- Next, the sections were dewaxed and rehydrated, and then antigen retrieval was performed by microwaving slides in citrate buffer at pH 6.0.
- Fifty-micrometer sections of paraffin-embedded, 10% neutral buffered formalin fixed tissues were dewaxed and rehydrated, and a whole nuclei prep was made by digesting with trypsin.
- These sections were then dewaxed in xylene and rehydrated in graded ethanols.
- For DNA extraction, three 10 m sections were cut from paraffin blocks, dewaxed and dried in a vacuum.
- After dewaxing in xylene and ethanol, the tissue was fixed in 2.5% glutaraldehyde followed by 1% osmium tetroxide.
- Sections from selected formalin-fixed and paraffin-embedded tissue blocks were placed on coated slides, dewaxed and rehydrated routinely, and then washed in TdT buffer.
- Before immunostaining, the slides were dewaxed and endogenous peroxidase activity was blocked with 0.45% hydrogen peroxide in 99% ethanol for 15 minutes.
- Slides were dewaxed, then incubated for 30 min in proteinase K at 37°C.
- For immunostaining, the sections were mounted on sialinated slides, dewaxed, and hydrated.
- Sections were dewaxed in xylene and hydrated in ethanol.
- Tissue sections were cut at 5 m, dewaxed, and hydrated in xylene and ethanol before antigen retrieval.
- Two sections of each specimen were dewaxed in xylene for 10 minutes and rinsed thoroughly in absolute ethanol twice.
- Genomic DNA was extracted by dewaxing with xylene, proteinase K digestion, and phenol/chloroform extraction.
- Briefly, the sections were dewaxed and then placed in 30% hydrogen peroxide solution to block endogenous peroxidase.
- To determine the net orientation of the cellulose wall microfibrils the longitudinal sections were dewaxed, mounted in DPX and examined under polarized light.
- Five-micrometer sections of paraffinembedded tissues were dewaxed, and antigen was retrieved by microwaving in 0.1M Tris buffer containing 0.15M sodium chloride and 0.1M potassium chloride at pH 7.4.
- For M30 immunostaining, sections were cut, dewaxed, and hydrated as described herein.
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