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词汇 Sepharose
释义

Definition of Sepharose in English:

Sepharose

noun ˈsɛfərəʊzˈsefəˌrōs
mass nountrademark

  • A preparation of agarose used as a gel in chromatography, electrophoresis, and other separation techniques.

    〈商标〉琼脂糖（凝胶）

    Example sentencesExamples
    • The mutant protein was immobilized on CNBr-activated Sepharose according to the manufacturer's instruction.
    • The purification of VAA started from aqueous extracts of dried mistletoe leaves and also used affinity chromatography on lactosylated Sepharose as the crucial step as previously described.
    • One hundred microliters of 10% (w/v) protein-A Sepharose in lysis buffer was added and mixed at 4° for 1 hr.
    • Polyclonal anti-RIP1 antibody was purified over a column of recombinant His-tagged proRIP1 coupled to cyanogen-bromide activated Sepharose.
    • After 1 hr incubation at room temperature, labeled proteins were recovered by binding to protein A Sepharose and washing extensively in RIPA buffer as well as 100 mM Tris, pH 7.5, supplemented with NP - 40 (nonionic detergent).

Origin

1960s: of unknown origin.

Definition of Sepharose in US English:

Sepharose

nounˈsefəˌrōs
trademark

  • A preparation of agarose used as a gel in chromatography, electrophoresis, and other separation techniques.

    〈商标〉琼脂糖（凝胶）

    Example sentencesExamples
    • The mutant protein was immobilized on CNBr-activated Sepharose according to the manufacturer's instruction.
    • One hundred microliters of 10% (w/v) protein-A Sepharose in lysis buffer was added and mixed at 4° for 1 hr.
    • Polyclonal anti-RIP1 antibody was purified over a column of recombinant His-tagged proRIP1 coupled to cyanogen-bromide activated Sepharose.
    • After 1 hr incubation at room temperature, labeled proteins were recovered by binding to protein A Sepharose and washing extensively in RIPA buffer as well as 100 mM Tris, pH 7.5, supplemented with NP - 40 (nonionic detergent).
    • The purification of VAA started from aqueous extracts of dried mistletoe leaves and also used affinity chromatography on lactosylated Sepharose as the crucial step as previously described.

Origin

1960s: of unknown origin.
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