Plasmids were transformed into DH5 using the Hanahan procedure or by electroporation at 2.5 mV using a Bio-Rad (Hercules, CA) E. coli pulser.
A total of 1 l of ligation product was used to transform competent Escherichia coli cells by electroporation using a Bio-Rad (Richmond, CA) gene pulser.
Host cells were transformed under the following parameters for BIORAD gene pulser and gene controller: 1250 V - 25 F - 200 [Omega]. Clones were isolated by plating on selective media.
MT + or clone B CHO cells were resuspended in 800 l of phosphate buffered saline and electroporated with 2.5 g of pTNeo 99-7 using a Bio-Rad gene pulser.
Gate pulses of 10 ns duration were delivered by a high-voltage fast pulser.
Definition of pulser in US English:
pulser
nounˈpəlsərˈpəlsər
See pulse
Example sentencesExamples
MT + or clone B CHO cells were resuspended in 800 l of phosphate buffered saline and electroporated with 2.5 g of pTNeo 99-7 using a Bio-Rad gene pulser.
Plasmids were transformed into DH5 using the Hanahan procedure or by electroporation at 2.5 mV using a Bio-Rad (Hercules, CA) E. coli pulser.
Host cells were transformed under the following parameters for BIORAD gene pulser and gene controller: 1250 V - 25 F - 200 [Omega]. Clones were isolated by plating on selective media.
A total of 1 l of ligation product was used to transform competent Escherichia coli cells by electroporation using a Bio-Rad (Richmond, CA) gene pulser.
Gate pulses of 10 ns duration were delivered by a high-voltage fast pulser.