Four lysogens (HI2678, HI2679, HI2680, and HI2681) were prepared by transformation of the wild-type strain or ligts7 mutant strain with the high-copy-number plasmids pSY343 or pLP312.
These encode resistance to the antibiotic, allowing selection of lysogens in a wide variety of strains, including those resistant to the antibiotics tetracycline, ampicillin, kanamycin, and chloramphenicol.
The tentative map location of the suppressor gene or of the nearby Tn10dTc element was determined using the Mud-P22 lysogen collection of BENSON and GOLDMAN 1992.
The ratio of the titer on BB to that on 594 was then used as a plating efficiency quotient to correct the titers of the recombinants observed on the given lysogen.
Definition of lysogen in US English:
lysogen
nounˈlīsəjən
A lysogenic bacterium or bacterial strain.
Example sentencesExamples
The tentative map location of the suppressor gene or of the nearby Tn10dTc element was determined using the Mud-P22 lysogen collection of BENSON and GOLDMAN 1992.
The ratio of the titer on BB to that on 594 was then used as a plating efficiency quotient to correct the titers of the recombinants observed on the given lysogen.
Four lysogens (HI2678, HI2679, HI2680, and HI2681) were prepared by transformation of the wild-type strain or ligts7 mutant strain with the high-copy-number plasmids pSY343 or pLP312.
These encode resistance to the antibiotic, allowing selection of lysogens in a wide variety of strains, including those resistant to the antibiotics tetracycline, ampicillin, kanamycin, and chloramphenicol.