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词汇 ultracentrifuge
释义

Definition of ultracentrifuge in English:

ultracentrifuge

noun ʌltrəˈsɛntrɪfjuːdʒˌəltrəˈsɛntrəˌfjudʒ
  • A very fast centrifuge used to precipitate large biological molecules from solution or separate them by their different rates of sedimentation.

    超离心机

    Example sentencesExamples
    • The sarcosine insoluble fractions were removed by centrifugation at 100,000 g for 1 h in an ultracentrifuge, washed once with PBS, pH 7.4 and used as outer membrane protein fractions.
    • PLY solution species from monomer via multimeric intermediates to ring-shaped oligomers were studied with time-dependent sedimentation velocity in the analytical ultracentrifuge.
    • Boundary sedimentation and sedimentation equilibrium experiments were performed using an analytical ultracentrifuge Optima XL-I from Beckman Instruments (Palo Alto, CA) equipped with absorbance and interference optics.
    • After removing the cell debris by centrifugation, supernatant was layered on a 5.7 M CsCl / 0.01 M EDTA solution in an ultracentrifuge tube and centrifuged in a swinging bucket rotor at 40,000 rpm for 16 hr.
    • The supernatant was recentrifuged at 109,000 g on a tabletop ultracentrifuge (Rotor TLA 45 of Beckman centrifuges, Beckman Coulter, Fullerton, CA) for 1 h.
verbʌltrəˈsɛntrɪfjuːdʒˌəltrəˈsɛntrəˌfjudʒ
[with object]
  • Subject to the action of an ultracentrifuge.

    超速分离;用超离心机分离

    the samples were ultracentrifuged for two hours
    Example sentencesExamples
    • To concentrate the solution, the collagen was ultracentrifuged at 10 deg C for 26-48 It for preparation of 10-45 mg/ml gels.
    • The homogenate, obtained after sonication, was ultracentrifuged at 60 000 g for 30 min.
    • An additional 34 ml buffer solution was added and the sample was then ultracentrifuged at 100,000 × # for 4 h.
    • The remaining sample was ultracentrifuged to obtain the sputum sol phase.
    • The supernatant was ultracentrifuged at 105,000 × g, 4°C for 1.5 h.
    • The sample was then ultracentrifuged for 1 hour at 141,000 × g to remove membranes.

Derivatives

  • ultracentrifugal

  • adjective
    • One of the classical problems in the theory of ultracentrifugal sedimentation is the treatment of diffusion in sizedistribution analysis.
      Example sentencesExamples
      • I used to run models of the ultracentrifugal rocket engine Roger Gregory and I designed.
  • ultracentrifugation

  • noun
    • This was shown in the earlier analytical ultracentrifugation analyses comparing DNA content from thermally ruptured phage (when all of the DNA comes out) with that from samples treated with LamB with and without PEG.
      Example sentencesExamples
      • The total lysate separated into 4 different fractions whereas only the one with the lowest density was detected when the supernatant recovered after ultracentrifugation was loaded onto the sucrose gradient.
      • After ultracentrifugation, the free peptide concentration was determined fluorometrically.
      • To gain more insight into the salt-dependent behavior of SecB, we studied the protein by DLS, size exclusion chromatography, analytical ultracentrifugation, and small angle neutron scattering.
      • In the current context, for illustrating the behavior of the size distribution, we considered the data from a mixture of two different elution fractions after sucrosegradient ultracentrifugation.

Definition of ultracentrifuge in US English:

ultracentrifuge

nounˌəltrəˈsentrəˌfyo͞ojˌəltrəˈsɛntrəˌfjudʒ
  • A very fast centrifuge used to precipitate large biological molecules from solution or separate them by their different rates of sedimentation.

    超离心机

    Example sentencesExamples
    • The sarcosine insoluble fractions were removed by centrifugation at 100,000 g for 1 h in an ultracentrifuge, washed once with PBS, pH 7.4 and used as outer membrane protein fractions.
    • After removing the cell debris by centrifugation, supernatant was layered on a 5.7 M CsCl / 0.01 M EDTA solution in an ultracentrifuge tube and centrifuged in a swinging bucket rotor at 40,000 rpm for 16 hr.
    • The supernatant was recentrifuged at 109,000 g on a tabletop ultracentrifuge (Rotor TLA 45 of Beckman centrifuges, Beckman Coulter, Fullerton, CA) for 1 h.
    • Boundary sedimentation and sedimentation equilibrium experiments were performed using an analytical ultracentrifuge Optima XL-I from Beckman Instruments (Palo Alto, CA) equipped with absorbance and interference optics.
    • PLY solution species from monomer via multimeric intermediates to ring-shaped oligomers were studied with time-dependent sedimentation velocity in the analytical ultracentrifuge.
verbˌəltrəˈsentrəˌfyo͞ojˌəltrəˈsɛntrəˌfjudʒ
[with object]
  • Subject to the action of an ultracentrifuge.

    超速分离;用超离心机分离

    the samples were ultracentrifuged for two hours
    Example sentencesExamples
    • The sample was then ultracentrifuged for 1 hour at 141,000 × g to remove membranes.
    • An additional 34 ml buffer solution was added and the sample was then ultracentrifuged at 100,000 × # for 4 h.
    • The supernatant was ultracentrifuged at 105,000 × g, 4°C for 1.5 h.
    • To concentrate the solution, the collagen was ultracentrifuged at 10 deg C for 26-48 It for preparation of 10-45 mg/ml gels.
    • The homogenate, obtained after sonication, was ultracentrifuged at 60 000 g for 30 min.
    • The remaining sample was ultracentrifuged to obtain the sputum sol phase.
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